THE TECHNIQUE OF STAINING LEPROSY BACILLI IN SMEARS

Improved staining of leprosy bacilli in tissues.

A technique which reliably demonstrates Mycobacterium leprae, M. tuberculosis and fungi in tissues is described. It is based on the oxidation of cell wall lipid substances by chromic or periodic acid, and the subsequent release of aldehydes which are then capable of reducing ammoniacal silver salt solutions to metallic silver. The organisms so demonstrated appear uniformly solid. The sensitivit...

A new method for staining leprosy bacilli.

To a paper on a new method for staining tubercle bacilli with Nachtblau which the writer published in 1941, Reenstierna added a short note (1), together with a plate, on its use in staining leprosy bacilli in nasal smears, films of blood, and sections of lepromata. As was the case with tubercle bacilli, the bacilli of leprosy were found to take a dark-blue color, to appear larger and more disti...

A Study of Malachite Green Staining of Leprosy Bacilli.

Comparison of Leishman Staining With H&E Staining Technique in the Study of FNAC Smears

Introduction:Fine Needle Aspiration Cytology (FNAC) is used widely for the preliminary pre-operative diagnosis of all superficial palpable lesions and for deep seated lesions under ultrasound guidance. Though Pap stain, May GrunwaldGiemsa (MGG) and Haematoxylin and Eosin (H & E) stains are used commonly, Leishman stain provides good cytoplasmic and background details. Hence, it can beused in ad...

Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy.

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears ...